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目的探讨流感嗜血杆菌肺炎患儿免疫功能。方法对入院儿童行多病原联合检测,明确流感嗜血杆菌感染,并用流式细胞仪检测患儿外周血T细胞亚群、B细胞及NK细胞。结果肺炎组患儿T细胞亚群(CD3+CD4+T,CD3+CD8+T)与对照组比较,差异均无统计学意义(P>0.05);而B细胞(CD3-CD19+B)比例较对照组增高,差异有统计学意义(P<0.05);NK细胞(CD3-CD16+CD56+NK)较对照组下降,差异有高度统计学意义(P<0.01)。结论流感嗜血杆菌肺炎患儿体液免疫反应增强,固有免疫减弱。感染后免疫功能测定和分析有利于临床免疫调节治疗,对病情及预后的判断有一定的指导意义。
Objective To investigate the immune function of children with Haemophilus influenzae pneumonia. Methods Combined detection of multi-pathogenic bacteria in children admitted to hospital confirmed the infection of Haemophilus influenzae. The T lymphocyte subsets, B cells and NK cells in peripheral blood of children were detected by flow cytometry. Results There were no significant differences in T cell subsets (CD3 + CD4 + T, CD3 + CD8 + T) in children with pneumonia compared with the control group (P> 0.05) (P <0.05); NK cells (CD3-CD16 + CD56 + NK) decreased compared with the control group, the difference was statistically significant (P <0.01). Conclusion The humoral immune response in children with Haemophilus influenzae pneumonia is enhanced and the innate immunity is weakened. Immune function determination and analysis after infection is beneficial to the clinical immunomodulation therapy, which is of guiding significance for the judgment of disease and prognosis.