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目的:探索山莨菪碱对肝脏再灌注后肝组织中ATP和肝细胞内游离Ca~(2+)的影响。 方法:选用(?)wistar大鼠30只,分为正常对照组,缺血再灌注组和山莨菪碱组。观察大鼠缺血60min再灌注1h后肝组织中三磷酸腺苷(ATP),丙二醛(MDA)和肝细胞内游离钙([Ca~(2+)]i)含量的变化,同时观察肝组织病理学变化。 结果:肝脏缺血再灌注后肝组织中MDA和肝细胞内[Ca~(2+)]i含量显著增加,而肝组织中ATP含量明显降低,应用山莨菪碱后,肝组织中MDA和肝细胞内[Ca~(2+)含量降低,而肝组织中ATP含量下降不明显,同时肝组织病理学损害减轻。 结论:山莨菪碱可提高缺血再灌后肝组织中ATP含量,减少Ca~(2+)在肝细胞内的蓄积,从而对肝脏缺血再灌注损伤具有一定的保护作用。
Objective: To explore the effect of anisodamine on ATP and free intracellular Ca 2+ in hepatic tissue after liver reperfusion. Methods: Thirty Wistar rats were divided into normal control group, ischemia-reperfusion group and anisodamine group. The changes of ATP, MDA and [Ca ~ (2 +)] i in liver were observed after ischemia 60 min and reperfusion 1 hour, Neo-Confucianism changes. Results: The content of MDA and the content of [Ca ~ (2 +)] i in hepatic tissue increased significantly after hepatic ischemia-reperfusion, while the content of ATP in hepatic tissue decreased obviously. After application of anisodamine, MDA and liver The content of Ca2 + in the cells decreased, but the content of ATP in the liver tissue did not decrease obviously, and the pathological damage of the liver tissue was alleviated. Conclusion: Anisodamine can increase the content of ATP in the liver tissue after ischemia - reperfusion and decrease the accumulation of Ca ~ (2 +) in hepatocytes, which may have a protective effect on hepatic ischemia - reperfusion injury.