论文部分内容阅读
目的通过观察通心络胶囊对载脂蛋白E(ApoE)基因敲除小鼠循环内皮细胞(CEC)、小鼠内皮素(ET)水平的干预变化,探究其对ApoE基因敲除小鼠冠状动脉粥样硬化(AS)的调节作用和机理。方法将40只ApoE基因敲除小鼠作为实验组,建立冠状AS模型,随机分为模型组等5组。结果通心络胶囊干预后,通心络胶囊高、中、低剂量组和辛伐他汀组、模型组相比,通心络胶囊高剂量组在造模后第4、8、12周血中CEC均明显低于模型组,中剂量组在造模后第4周和第12周时血中CEC与模型组相比显著降低。模型组小鼠血清ET含量高于正常组,通心络胶囊各剂量组小鼠血清ET含量均低于模型组。结论通心络胶囊能降低ApoE基因敲除小鼠CEC、ET水平,具有调节血管内皮细胞功能和抑制炎症因子的作用。
Objective To observe the intervention of Tongxinluo capsule on the level of circulating endothelial cell (CEC) and mouse endothelin (ET) in apolipoprotein E (ApoE) knockout mice and to explore its effect on the coronary artery of ApoE knockout mice Regulatory effect and mechanism of atherosclerosis (AS). Methods Forty ApoE knockout mice were used as the experimental group. Coronary AS model was established and randomly divided into 5 groups: model group. Results Tongxinluo capsule intervention, Tongxinluo capsule high, medium and low dose groups and simvastatin group, model group, Tongxinluo capsule high-dose group in the model after 4,8,12 weeks of blood CEC were significantly lower than the model group, the middle dose group in the 4th and 12th week after modeling in the blood CEC was significantly lower than the model group. The content of serum ET in model group was higher than that in normal group. The content of ET in serum of Tongxinluo capsule group was lower than model group. Conclusion Tongxinluo Capsule can reduce the level of CEC and ET in ApoE knockout mice, regulate the function of vascular endothelial cells and inhibit the inflammatory cytokines.