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利用Ｌａｎｇｅｎｄｏｒｆｆ方法灌注心脏，观察了离体大鼠心脏再灌注后心肌特异性肌钙蛋白Ｔ（ＣａｒｄｉａｃｓｐｅｃｉｆｉｃｔｒｏｐｏｎｉｎＴ，ＴｎＴ）、磷酸肌酸激酶（ＣＫ）、乳酸脱氢酶（ＬＤＨ）的释放过程及其与左室功能的关系。结果显示：（１）ＴｎＴ呈双峰曲线，再理注后ＴｎＴ迅速上升，５分钟后下降，然后又逐渐升高，４小时仍保持较高水平。ＣＫ、ＬＤＨ呈单峰曲线，再灌注５分钟ＣＫ、ＬＤＨ达高峰，其后逐渐下降，３～４ｈ恢复至基线水平。（２）４小时内ＬＤＨ、ＴｎＴ的总释放量（ΣＬＤＨ、ΣＴｎＴ）与左室压力（ＬＶＰ）呈负相关（ｔ分别为－０．８３、－０．７１，Ｐ均＜０．００２），ΣＣＫ与ＬＶＰ无相关关系。（３）再灌注４小时，１分钟内ＴｎＴ的释放量与ＬＶＰ呈负相关（ｒ＝－０．６８，Ｐ＜０．００２），而ＣＫ、ＬＤＨ与ＬＶＰ无相关关系。结果表明，ＴｎＴ可以作为判定心肌细胞坏死程度及左室功能障碍的良好指标，优于传统心肌酶学测定。
Cardiac specific troponin T (TnT), creatine phosphokinase (CK) and lactate dehydrogenase (LDH) release were measured by Langendorff perfusion in vitro. Left ventricular function. The results showed that: (1) TnT showed a bimodal curve. After reperfusion, the TnT increased rapidly, then decreased after 5 minutes, and then gradually increased. It remained at a high level after 4 hours. CK, LDH showed a single peak curve, 5 minutes after reperfusion, CK, LDH reached its peak, then gradually decreased 3 ~ 4h returned to baseline levels. (2) The total release of LDH and TnT (ΣLDH, ΣTnT) was negatively correlated with left ventricular pressure (LVP) within 4 hours (t = -0.83, -0.71, P <0.002 respectively) ΣCK and LVP no correlation. (3) After 4 hours of reperfusion, the release of TnT in one minute was negatively correlated with LVP (r = -0.68, P <0.002), while there was no correlation between CK, LDH and LVP. The results show that, TnT can be used as a good indicator of myocardial cell necrosis and left ventricular dysfunction, better than the traditional myocardial enzymometry.