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目的:研究益气养阴方及其拆方后的扶正方、祛邪方对细胞周期及相关蛋白p21在人急性髓细胞白血病(acutemyeloid leukemia,AML)中表达的影响,探讨益气养阴方治疗白血病的作用机制。方法:NOD-SCID小鼠经60Co照射后,尾静脉注射KG1a细胞1×106个,分成4组。对照组予生理盐水,实验组分别予益气养阴方、扶正方、祛邪方中药制剂灌胃。采用流式细胞术法检测细胞周期;免疫组化法检测p21蛋白的表达。结果:流式细胞术法检测细胞凋亡率:益气养阴组、扶正组、祛邪组与对照组比较,差异均有统计学意义(P<0.01)。免疫组化法检测c-myc、survivin蛋白的表达:对照组与益气养阴组、扶正组、祛邪组比较差异有统计学意义(P<0.05)。结论:中药益气养阴方可升高p21蛋白的表达,诱导白血病细胞停滞在G1期,对AML治疗具有作用。
Objective: To study the effect of Qiqiyangyin Decoction and Fuzheng Formula and Quxie Recipe on the expression of cell cycle and related protein p21 in acute myeloid leukemia (AML) and explore the effects of Yiqi Yangyin Decoction Treatment mechanism of leukemia. METHODS: After irradiation with 60Co NOD-SCID mice, 1 x 106 KG1a cells were injected into the tail vein and divided into 4 groups. The control group was given normal saline, and the experimental group was given intragastric administration of traditional Chinese medicine preparations of Yiqiyangyin Decoction, Fuzheng Fang, and Decoction. The cell cycle was detected by flow cytometry, and the expression of p21 protein was detected by immunohistochemistry. RESULTS: The apoptosis rate of cells was detected by flow cytometry: There were significant differences between the Yiqiyangyin group, the Fuzheng group and the Shuxie group compared with the control group (P<0.01). Immunohistochemical method was used to detect the expression of c-myc and survivin proteins: There were significant differences between the control group and the Yiqiyangyin group, the Fuzheng group and the Shuxia group (P<0.05). Conclusion: The traditional Chinese medicine Yiqi Yangyin Decoction can increase the expression of p21 protein and induce the arrest of leukemia cells in G1 phase, which has an effect on the treatment of AML.