论文部分内容阅读
[目的]探讨宫颈鳞状细胞癌组织中p27和CyclinE的表达及其与临床病理因素和预后的关系。[方法]采用免疫组化二步法检测70例宫颈鳞癌(CSES)、30例宫颈鳞状上皮内瘤变(CIN)以及正常宫颈组织(NCE)中p27和CyclinE的表达,并分析两者的表达与CSEC患者临床病理因素及预后的关系。[结果]p27蛋白在CSEC、CIN和NCE中的阳性表达率分别为42.9%、76.7%和96.0%;而CyclinE的阳性表达率则分别为64.3%、26.7%和6.0%。p27和CyclinE蛋白表达均与组织学分级、间质浸润、淋巴结转移以及脉管浸润有关(P<0.05),两者的表达呈显著负相关(r=-0.504,P<0.01)。[结论]CyclinE高表达伴p27低表达提示肿瘤的高侵袭性及不良预后。
[Objective] To investigate the expression of p27 and CyclinE in cervical squamous cell carcinoma and its relationship with clinicopathological factors and prognosis. [Method] The expression of p27 and CyclinE in 70 cases of cervical squamous cell carcinoma (CSES), 30 cases of cervical squamous intraepithelial neoplasia (CIN) and normal cervical tissue (NCE) were detected by immunohistochemical two-step method. And its relationship with clinicopathological factors and prognosis in patients with CSEC. [Results] The positive expression rates of p27 protein in CSEC, CIN and NCE were 42.9%, 76.7% and 96.0% respectively, while the positive rates of CyclinE were 64.3%, 26.7% and 6.0% respectively. The expressions of p27 and CyclinE were correlated with histological grade, interstitial infiltration, lymph node metastasis and vascular infiltration (P <0.05). The expressions of p27 and CyclinE were negatively correlated (r = -0.504, P <0.01). [Conclusion] The high expression of CyclinE and the low expression of p27 suggest the high invasiveness and poor prognosis of the tumors.