Identification of Orientia tsutsugamushi,spotted fever group and typhus group Rickettsia by duplex a

• 作 者: M-C Luan D-Z Yu L Tang L-J Zha
• 机 构: DepartmentofRickettsiology,DepartmentofMicroorganism,DepartmentofMicroecology,DepartmentofRickettsio
• 出 处: 亚太热带医药杂志：英文版
• 发表日期: 2008年4期
• 关键词: Rickettsia GroEL GENE DUPLEX and nested PCR Rickettsia GroEL gene Duplex and nes
• 基金项目: supported by National Natural Science Foundation of China(No. 30771854),China-U.S.Collaborative Program on Emerging and Re-emerging Infectious Diseases(No. 1U2GGH000018-01)

【正】Objective:To identify members of genera of rickettsia and O.tsutsugammhi simultaneously.Methods:Rapid and duplex and nested PCR methods have been established by designing primers based on the conserved regions of heat shock protein GroEL gene.345 mouse