论文部分内容阅读
玻璃化冷冻保存技术由于具有操作简便、冻融损伤小并且不需要仪器精密控温的特点,已经成为最具应用前景的冷冻保存技术。玻璃化冷冻受到冷冻保护剂成分、浓度、作用时间、作用温度以及降温速率的影响。卵母细胞由于其特殊的细胞学特性,冷冻保存相对困难。玻璃化冻存技术应用于卵母细胞的冷冻保存能获得较好的存活率、受精率以及卵裂率。但是,经过玻璃化冻融的卵母细胞受精后囊胚形成率还不理想,高浓度的冷冻保护剂是否具有细胞毒性,是否会影响胚胎发育等问题尚待解答。因此,还需要更深入的研究来改善卵母细胞的玻璃化冷冻保存技术。
Vitrification cryo-preservation technology has become the most promising cryopreservation technology because of its simple operation, small freeze-thaw damage and lack of precise temperature control of the instrument. Vitrification is affected by the cryoprotectant composition, concentration, duration of action, temperature of action, and rate of temperature decrease. Oocytes are relatively difficult to cryopreservation due to their special cytological properties. Vitrification cryopreservation technology used in oocyte cryopreservation can get better survival rate, fertilization rate and cleavage rate. However, the rate of blastocyst formation after fertilization of vitrified freeze-thawed oocytes is not ideal, and whether the high concentration of cryoprotectant is cytotoxic or not affects embryo development remains to be answered. Therefore, more research is needed to improve the vitrification of oocytes.