论文部分内容阅读
目的研究人类新基因nm23-H1B与卵巢肿瘤的关系。方法收集2003年4月~2004年2月手术切除各种卵巢肿瘤标本48例,正常卵巢组织8例。采用RT-PCR、Northern杂交、原位杂交检测nm23-H1BmRNA的表达。结果用RT-PCR在所有的标本中均检测到nm23-H1B基因mRNA的表达,其在正常卵巢组织中表达较低,而在所有的卵巢肿瘤中表达均出现明显上调,在早期卵巢癌中的表达高于晚期。Northern杂交显示nm23-H1B基因在正常卵巢组织的表达量较低,交界性肿瘤中的表达则与正常组织中相似,而在卵巢癌组织中表达明显增高,在早期卵巢癌中在分化好的组织中(Ⅰ级)表达量明显高于分化较差(Ⅱ、Ⅲ级)者。原位杂交显示nm23-H1B基因在正常卵巢组织和交界性肿瘤的表达量极低,而在卵巢癌组织中表达明显增高,mRNA表达产物主要位于细胞质内;早期卵巢癌中的mRNA表达量高于晚期卵巢癌。结论nm23-H1B基因与卵巢癌有明显的相关性。
Objective To study the relationship between the human new gene nm23-H1B and ovarian tumors. Methods 48 cases of ovarian tumor specimens were collected from April 2003 to February 2004, and 8 cases of normal ovarian tissues were collected. The expression of nm23-H1B mRNA was detected by RT-PCR, Northern blot and in situ hybridization. Results The expression of nm23-H1B mRNA was detected by RT-PCR in all samples, which was lower in normal ovarian tissues and significantly up-regulated in all ovarian tumors. In early stage of ovarian cancer Expression higher than late. Northern blot showed that the expression of nm23-H1B gene in normal ovarian tissue was low, and the expression in borderline tumors was similar to that in normal tissues, while it was significantly increased in ovarian cancer tissues. In early stage ovarian cancer, the differentiated tissues (Ⅰ grade) was significantly higher than the poorly differentiated (Ⅱ, Ⅲ grade). In situ hybridization showed that the expression of nm23-H1B gene in normal ovarian tissues and borderline tumors was extremely low, while it was significantly increased in ovarian cancer tissues. The mRNA expression products were mainly located in the cytoplasm. The mRNA expression of nm23- Advanced ovarian cancer. Conclusion There is a clear correlation between nm23-H1B gene and ovarian cancer.