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用35个镜鲤微卫星QTL标记评估了豫选黄河鲤(Cyprinus carpio var.haematopterus)群体的遗传结构,并评估了不同基因型与生长性状(体重、体长、体高和体厚)的相关性,筛选了在群体中具有性状优势的基因型。35个微卫星标记在288个豫选黄河鲤个体中的扩增结果显示,各标记等位基因数为3~13,平均每个标记6.828 6个;平均有效等位基因数为4.520 8;平均观测杂合度为0.603 0;平均期望杂合度为0.701 9;平均多态信息含量为0.665 6,群体整体处于高度多态水平(PIC>0.5)。利用SPSS19.0的GLM模型分析标记与性状的相关性,共17个微卫星标记与性状表现出不同程度的相关性,其中HLJ2456、HLJ2387和CA1677 3个标记与相应的性状相关性达极显著水平。用Duncan氏多重比较找到了每个微卫星标记具有生长性状优势的基因型,下一步可根据优势基因型指导豫选黄河鲤家系配组,开展基于QTL结果的分子聚合育种研究。
The genetic structure of the population of Cyprinus carpio var. Haematopterus was evaluated using 35 microsatellite QTL markers of mirror carp and the correlation between different genotypes and growth traits (body weight, body length, body height and body thickness) was evaluated , Screened genotypes with trait predominance in the population. The results of amplification of 35 microsatellite markers in 288 individuals of Yuyi Yellow River carp showed that the number of alleles of each marker ranged from 3 to 13 with an average of 6.828 per marker. The average effective number of alleles was 4.520 8. The mean The observed heterozygosity was 0.603 0. The average expected heterozygosity was 0.701 9. The average polymorphism information content was 0.665 6. The population was highly polymorphic (PIC> 0.5). The correlation between markers and traits was analyzed by GLM model of SPSS19.0. A total of 17 microsatellite markers showed different degrees of correlation with traits. Among them, HLJ2456, HLJ2387 and CA1677 showed significant correlation with their traits . Using Duncan’s multiple comparisons to find out the genotypes with microsatellite markers for their growth traits. The next step is to guide the selection of the Yellow River carp family members based on the dominant genotype to carry out the study of molecular polymerization breeding based on the QTL results.