论文部分内容阅读
目的确定新西兰小鼠自身抗染色质抗体的遗传易感基因染色体定位。方法建立新西兰黑色品系(NZB)与新西兰白色品系(NZW)F1×NZB回交小鼠模型。采用覆盖小鼠19条染色体的微卫星遗传标记及数量性状位点(QTL)分析进行基因定位。结果确定了自身抗染色质抗体产生的两个遗传易感基因,一个位于NZW小鼠第9染色体中段30厘摩临近;另一个易感基因位于NZW小鼠第17染色体近中心端19厘摩处(Lods值>3)。结论(NZB×NZW)F1小鼠自身抗染色质抗体的产生受多基因调控,候选易感基因不仅来自于NZB,也来自于NZW。
OBJECTIVE: To determine the chromosomal location of the genetic predisposition genes of autoantibodies against chickens in New Zealand mice. Methods The F1 × NZB backcross mice model was established between New Zealand Black Line (NZB) and New Zealand White Line (NZW). Microsatellite markers and quantitative trait loci (QTLs) were used to detect the 19 chromosomes in mice. The results identified two genetically susceptible genes produced by the autoantibodies, one approaching 30 centimeters in the middle of chromosome 9 in NZW mice and another 19 locus in the proximal center of chromosome NZW in NZW mice (Lods value> 3). Conclusion The production of autoantibodies to autoantibodies in F1 (NZB × NZW) mice is regulated by polygenes. The candidate susceptible genes are not only derived from NZB but also from NZW.