论文部分内容阅读
目的研究联合使用碱性成纤维细胞生长因子(basicfibroblastgrowthfactor,bFGF)和三磷酸腺苷(adenosinetri2phosphate,ATP)对体外培养的乳鼠脊髓神经元的作用。方法将不同的干预物加入培养基,分为bFGF组,ATP组,ATP+bFGF组以及单纯对照组。相差倒置显微镜观察细胞生长情况,计数绘制细胞生长曲线,并测量细胞突起的长度;用MTT法测定培养细胞的存活率。结果(1)细胞突起长度:实验各组神经元轴突的长度均长于对照组(P<0.01)。接种48h和96h后,ATP+bFGF组细胞突起长度明显长于ATP组(t=6.576、7.032,P<0.01);ATP+bFGF组与bFGF组相比无差异(t=2.543、3.195,P>0.05);bFGF组细胞突起长度明显长于ATP组(t=6.245、8.026,P<0.01)。(2)比较MTT值:实验各组的灰度均明显大于对照组(P<0.01)。ATP+bFGF组的灰度明显大于ATP组(t=6.053,P<0.01)。ATP+bFGF组的灰度明显大于bFGF组(t=4.971,P<0.01)。bFGF组与ATP组相比无差异(P>0.05)。结论ATP、bFGF对于体外培养的脊髓神经元的存活均有较强的维持作用,并能促进轴突生长;两者联合使用作用明显增强。
Objective To study the effect of basic fibroblast growth factor (bFGF) and adenosine triphosphate (ATP) on the cultured spinal cord neurons in vitro. Methods Different interventions were added to the culture medium and divided into bFGF group, ATP group, ATP + bFGF group and simple control group. Inverted microscope was used to observe the cell growth. The cell growth curve was counted and the length of the cell protrusion was measured. The survival rate of the cultured cells was determined by MTT assay. Results (1) Cell protrusion length: The lengths of axons in neurons in each group were longer than those in the control group (P <0.01). The length of protuberances in ATP + bFGF group was significantly longer than that in ATP group at 48h and 96h after inoculation (t = 6.576, 7.032, P <0.01). There was no difference between ATP + bFGF group and bFGF group (t = 2.543,3.195, P> 0.05 ). The length of the cell protrusion in bFGF group was significantly longer than that in ATP group (t = 6.245, 8.026, P <0.01). (2) Compare the MTT value: The gray value of each group in the experiment is obviously larger than the control group (P <0.01). The gray scale of ATP + bFGF group was significantly greater than that of ATP group (t = 6.053, P <0.01). The gray scale of ATP + bFGF group was significantly greater than that of bFGF group (t = 4.971, P <0.01). There was no difference between bFGF group and ATP group (P> 0.05). Conclusions ATP and bFGF can maintain the survival of spinal cord neurons cultured in vitro and promote the growth of axons. The combined effect of ATP and bFGF can be significantly enhanced.