论文部分内容阅读
旨在评估茶氨酸(T)和本实验室合成的新颖的茶氨酸衍生物-茶氨酸氯香酰胺(TClC)对高转移的人乳腺癌MDA-MB-231细胞生长的抑制作用,并对其作用的机制进行初步探究。采用MTT法检测不同浓度的T和TClC对MDA-MB-231细胞体外生长的影响,采用Western blotting对MDA-MB-231细胞中与癌细胞凋亡相关蛋白的表达以及药物作用可能的分子靶点进行检测。结果显示,随着浓度的增高,T和TClC对人乳腺癌MDA-MB-231细胞体外生长的抑制作用逐渐增强,TClC的抑制作用要明显强于T;T和TClC均能够下调抗凋亡蛋白Bcl-2的表达水平,同时上调促凋亡蛋白Bax的表达水平,使Bcl-2/Bax比率减少。此外,T和TClC均能抑制血管内皮生长因子受体VEGFR1和核转录因子NF-κB的表达,而TClC的抑制作用要明显强于T。T和TClC对这些蛋白水平的调节作用可能是抑制MDA-MB-231细胞生长的重要机制之一。这些结果表明,T和TClC对治疗高转移的人乳腺癌可能具有广阔的应用前景。
The purpose of this study was to evaluate the inhibitory effect of theanine (T) and the novel theanine derivative, theanine chloramide (TClC) synthesized by our laboratory, on the growth of highly metastatic human breast cancer MDA-MB-231 cells. And the mechanism of its role to conduct a preliminary inquiry. The effect of different concentrations of T and TClC on the growth of MDA-MB-231 cells in vitro was detected by MTT assay. The expression of apoptosis related proteins in MDA-MB-231 cells and the possible molecular targets of drug action were detected by Western blotting Test. The results showed that the inhibitory effect of T and TClC on the growth of human breast cancer MDA-MB-231 cells gradually increased with the increase of concentration, and the inhibitory effect of TClC was stronger than that of T; both T and TClC downregulated the anti-apoptotic protein Bcl-2 expression levels, while increasing the expression of pro-apoptotic protein Bax, Bcl-2 / Bax ratio decreased. In addition, both T and TClC inhibited the expression of vascular endothelial growth factor receptor VEGFR1 and nuclear transcription factor NF-κB, while the inhibitory effect of TClC was stronger than that of T and TClC. The regulatory effect of T and TClC on these protein levels may be one of the important mechanisms that inhibit the growth of MDA-MB-231 cells. These results indicate that T and TClC may have broad application prospects for the treatment of highly metastatic human breast cancer.