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柑橘叶斑驳病毒(Citrus leaf blotch virus,CLBV)为柑橘病毒属(Citrivirus)代表种。本研究采用RT-PCR技术从我国栽培猕猴桃上首次检测到CLBV,总检出率为13.3%。测定了5个CLBV分离物的外壳蛋白基因(coat protein gene,cp)序列,全长均为1 092核苷酸(nt),分离物间cp基因核苷酸和编码氨基酸序列相似性分别为83.2%~99.7%和91.9%~98.9%,这些分离物与新西兰报道的CLBV猕猴桃分离物M3-A核苷酸序列相似性仅为83%左右,与来源于柑橘及近缘种的CLBV分离物cp基因核苷酸序列相似性为84%~86%。在基于该病毒cp基因核苷酸序列的系统进化树中,本研究所测定的CLBV猕猴桃分离物与新西兰报道的除M3-A外的猕猴桃分离物聚在同一组群。研究结果为进一步明确CLBV在我国猕猴桃上的侵染和危害特点及建立该病毒的分子检测技术提供了重要信息。
Citrus leaf blotch virus (CLBV) is a citrus genus representative. In this study, CLBV was first detected by RT-PCR from cultivated kiwifruit in China with a total detection rate of 13.3%. The coat protein gene (cp) sequences of five CLBV isolates were detected, and the total length of the coat protein gene (cp) was 1 092 nucleotides (nt). The nucleotide and amino acid sequence identities of the isolated cp genes were 83.2 % ~ 99.7% and 91.9% ~ 98.9%, respectively. The similarity of these isolates to the M3-A nucleotide sequence of CLBV kiwifruit isolate reported in New Zealand was about 83%, which was similar to that of CLBV isolates from citrus and related species cp Gene nucleotide sequence similarity of 84% ~ 86%. In the phylogenetic tree based on the nucleotide sequence of the viral cp gene, the CLBV kiwifruit isolate assayed in this study is clustered with the kiwifruit isolate reported in New Zealand excluding M3-A. The results provide important information for further clarifying the characteristics of CLBV infection and harm in Chinese kiwifruit and establishing the molecular detection technology of the virus.