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We used whole-vacuolar patch-clamp recording mode to study the action mechanism of La3+ to Slow Vacuolar (SV) channels for the first time. We recorded SV channel currents of Xinlimei (Raphanus satirus L.) vacuolars. The minimum activation potentials of voltage-dependent SV channels lied in 25±5 mV. The increase in cytoplasmic Ca2+ led to enhancement of SV-type currents. It was found that the threshold potential of activation shifted towards more depolarized values whenever cytoplasmic Ca2+ was increased. When 10-10 mol/L free La3+ was added to the bath, SV-type current was suppressed by 60~75%. These data showed La3+ reduced ion permeabilities of Xinlimei root vacuolar membrane.
We used whole-vacuolar patch-clamp recording mode to study the action mechanism of La3 + to Slow Vacuolar (SV) channels for the first time. We recorded SV channel currents of Xinlimei (Raphanus satirus L.) vacuolars. The minimum activation potentials of voltage It was found that the threshold potential of activation shifted towards more depolarized values whenever cytoplasmic Ca2 + was increased. When 10-10 mol / L free La3 + was added to the bath, SV-type current was suppressed by 60-75%. These data showed La3 + reduced ion permeabilities of Xinlimei root vacuolar membrane.