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本文报道化学致癌物二亚硝基哌嗪（DNP）诱发转基因小鼠鼻咽上皮细胞体外恶性转化。转化细胞表现为形态学改变，生长寿命延长，停泊非依赖性生长，染色体异常，接种裸鼠后形成肿瘤。电镜观察这些细胞呈低分化鳞癌细胞特征，实验结果表明，体外培养的携带EB病毒潜伏膜蛋白瘤基因的转基因小鼠鼻咽上皮细胞在小鼠肝微粒体酶（S9mix）活化下，对DNP的转化具有一定的敏感性，
This article reports the malignant transformation of nasopharyngeal epithelial cells in vitro induced by the chemical carcinogen dinitrosopepiperazine (DNP). Transformed cells showed morphological changes, prolonged growth life, anchorage-independent growth, chromosomal abnormalities, tumor formation after inoculation of nude mice. Electron microscopy of these cells was poorly differentiated squamous cell carcinoma cell characteristics, the experimental results show that in vitro cultured EBV latent membrane protein tumor gene transgenic mouse nasopharyngeal epithelial cells in mouse liver microsomal enzyme (S9mix) activation, DNP The conversion has a certain sensitivity,