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目的：探讨原癌基因ｃ－ｆｏｓ在缺血再灌注肾脏中表达情况及其在急性缺血性肾脏损伤修复中的作用。方法：用免疫组织化学法及原位分子杂交技术，检测急性肾缺血再灌注后大鼠肾组织内Ｆｏｓ蛋白及ｃ－ｆｏｓｍＲＮＡ表达的分布、强度及时程动力学等指标。结果：缺血再灌注早期即可诱导肾组织中ｃ－ｆｏｓ的高效表达，且ｃ－ｆｏｓｍＲＮＡ表达早于Ｆｏｓ蛋白；Ｆｏｓ蛋白表达出现于再灌注后１ｈ，２～４ｈ达高峰，８ｈ开始锐减，２４ｈ消失；ｃ－ｆｏｓｍＲＮＡ再灌注后０．５ｈ出现表达，１ｈ达高峰，２ｈ开始锐减，４ｈ时几乎消失。Ｆｏｓ蛋白阳性分布主要集中于远曲小管、升支粗段及集合管细胞核内；ｃ－ｆｏｓｍＲＮＡ表达分布部位同Ｆｏｓ蛋白，但定位于细胞质内；而两者在肾小球内均无表达。结论：原癌基因ｃ－ｆｏｓ的高效表达可能参与缺血再灌注所致肾脏损伤修复的分子调控，从而影响其病理发生过程
Objective: To investigate the expression of proto-oncogene c-fos in ischemia-reperfusion kidneys and its role in the repair of acute ischemic kidney injury. Methods: Immunohistochemistry and in situ hybridization were used to detect the expression of Fos protein and c-fos mRNA in renal tissue after acute renal ischemia-reperfusion in rats. Results: The high expression of c-fos was induced in the early stage of ischemia-reperfusion and the expression of c-fos mRNA was earlier than that of Fos protein. The expression of Fos protein peaked at 1 h and reached the peak at 2 ~ 4 h after reperfusion, , 24h disappeared; c-fos mRNA expression appeared 0.5h after reperfusion, peaked at 1h, 2h began to drop, almost disappeared 4h. Fos protein positive distribution mainly in the distal convoluted tubule, ascending branch thick and collecting duct nucleus; c-fos mRNA expression and Fos protein distribution, but located in the cytoplasm; and both were not expressed in glomeruli. CONCLUSION: High expression of proto-oncogene c-fos may be involved in the molecular regulation of renal injury caused by ischemia-reperfusion and thus affect its pathological process