化疗及导向化疗治疗大肠癌与癌细胞凋亡研究

• 作 者: 卢献平 李宝金 陈声乐 吕斌 蒋宁一
• 机 构: 中山医科大学孙逸仙纪念医院
• 出 处: 世界华人消化杂志
• 发表日期: 1999年04期
• 关键词: 化疗治疗 大肠癌

目的研究化疗及导向化疗对大肠癌组织及细胞的影响．方法大肠腺癌患者３７例，根据是否注射化疗药物分成Ａ，Ｂ两组．Ａ组１７例，术前不注射任何化疗药物或化疗药物的偶联物，将其作为对照组；Ｂ组２０例，其中７例手术前３ｄｉｖ丝裂霉素Ｃ（ＭＭＣ８ｍｇ），１３例手术前ｉｖ导向化疗药物抗ＣＥＡ单克隆抗体－ＭＭＣ交联物（含ＭＭＣ８ｍｇ，抗ＣＥＡ单克隆抗体１０ｍｇ）；其后对所有３７例患者行手术治疗并切取标本进行病理组织学观察，ＡｇＮＯＲ颗粒检测及肿瘤浸润淋巴细胞分类及计数．结果Ａ组病理退变程度１７例（１０００％）均为１级；Ｂ组病理退变程度１级４例（２００％），２级１２例（６００％），３级４例（２００％），两组之间差异有高度显著性（Ｐ＝０００００００３７６）．Ａ组癌细胞ＡｇＮＯＲ颗粒／核均数为４１１颗；Ｂ组癌细胞ＡｇＮＯＲ颗粒／核均数为２５８颗，其差异有高度显著性（Ｐ＜００１，ｔ＝５１４３）．肿瘤浸润淋巴细胞以Ｔ细胞ＣＤ＋３为主，同组中Ｔ淋巴细胞ＣＤ＋３（ａＰ＜００１，ｔ＝２０２２９）与Ｂ淋巴细胞ＣＤ＋２０之间的差异有高度显著性（ｂＰ＜００１，ｔ＝１９７６１）；Ａ组Ｔ淋巴细胞ＣＤ＋３为６０９１％，Ｂ组Ｔ?

Objective To study the effect of chemotherapy and directional chemotherapy on colorectal cancer tissues and cells. Methods 37 patients with colorectal adenocarcinoma were divided into A and B groups according to whether they were injected chemotherapy drugs. In group A, 17 patients were given no chemotherapeutic or chemotherapeutic conjugates before the operation as a control group; 20 patients in group B, of whom 7 were preoperatively treated with 3 div mitomycin C (MMC 8 mg), and 13 were preoperatively Iv Chemotherapy-directed anti-CEA monoclonal antibody-MMC cross-linker (containing MMC 8 mg, anti-CEA monoclonal antibody 10 mg); thereafter all 37 patients were treated surgically and specimens were taken for histopathological observation, AgNOR particle detection and tumor Infiltrating lymphocyte classification and counting. Results The degree of pathological degeneration in group A was 100 cases (100%) were grade 1; the degree of pathological degeneration in group B was grade 1 in 4 cases (200%), grade 2 in 12 cases (600%), grade 3 In 4 cases (200%), the difference between the two groups was highly significant (P=0000000376). In group A, the average number of AgNOR particles/nucleus was 411; the average number of AgNOR particles/nucleus in group B was 2.58, which was highly significant (P<001, t=5143). ). The tumor-infiltrating lymphocytes were predominantly T-cell CD+3, and the difference between CD+3 (aP<001, t=20229) and B-cell CD+20 in the same group was highly significant (bP<001). , t = 19  761); A group of T lymphocytes CD +3 60  91%, B group T?