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目的:研究青蒿抗热应激作用及其机理。方法:利用高温高湿环境造成家兔热应激模型,观察青蒿总香豆素对家兔肛温上升及血液、肺组织磷脂酶A2活性和循环内皮细胞数目的变化,并测定其对脑、心脏、肾组织钠泵活性及小鼠抗脑缺氧能力的影响。结果:青蒿总香豆素可明显降低热应激家兔体温上升速度,并伴随血清、肺组织磷脂酶A2活性降低,循环内皮细胞减少,显著抑制脑、心、肾组织钠泵活性,延长断头小鼠张口喘气时间几近1倍。结论:香豆素部位可能是青蒿祛暑功效的活性成分群之一。
Objective: To study the anti-heat stress effect and mechanism of Artemisia annua. METHODS: Rabbit heat stress model was induced by high temperature and high humidity environment. The changes of arterial total coumarin on rabbit rectal temperature, phospholipase A2 activity and circulating endothelial cell number in blood and lung tissue were observed. The activity of sodium pump in heart, kidney and kidney tissues and the anti-brain hypoxia ability of mice. RESULTS: Total coumarin of Artemisia annua could significantly reduce the increase of body temperature in heat-stressed rabbits, accompanied with the decrease of phospholipase A2 activity in serum and lung tissue, and the decrease of circulating endothelial cells, which significantly inhibited the activity of sodium pump in brain, heart and kidney tissues. Maturation times in mice with head-to-mouth mice were nearly 1 times. Conclusion: The coumarin fraction may be one of the active ingredients of Artemisia annua.