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目的:研究鼠全脑缺血再灌注后c-Myc蛋白在海马回的表达。方法:健康雄性SD大鼠随机分为两组:缺血再灌注组(IR,n=30):采用4-VO法建立全脑缺血再灌注模型,6分钟缺血后给予再灌注。假手术组(PO,n=30):只暴露血管而不夹闭。各组动物6h、12h、24h、48h、72h、96h以4%多聚甲醛灌注处死,将脑组织切片进行免疫组化染色TUNEL和HE染色镜镜下观察海马回神经元形态结构改变及c-Myc在CA1和CA3区不同表达及阳性神经细胞数,进行统计学分析。结果:1全脑缺血再灌注后6h,c-Myc在IR组CA1区在有表达,24～48h表达强度增高,72h后强度下降,CA3区弱于CA1区,主要位于胞浆。2HE染色显示,全脑缺血再灌注后72h,IR组CA1区组织水肿明显,神经元数目减少,排列混乱,核膜不清,核仁消失,CA3区神经元改变较轻微。3TUNEL染色结果显示,IR组全脑缺血再灌注后24～48h后海马CA1区阳性细胞数最多,至再灌注后72h后,海马CA1区阳性细胞数减少。结论:c-Myc蛋白在全脑缺血再灌注后海马回CA1区及CA3区都有表达,只是强弱及细胞分布不同。
Objective: To investigate the expression of c-Myc protein in the hippocampus after global cerebral ischemia-reperfusion in rats. Methods: Healthy male Sprague-Dawley rats were randomly divided into two groups: Ischemia-reperfusion group (IR, n = 30): The model of global cerebral ischemia-reperfusion was established by 4-VO and reperfusion after 6 minutes. Sham operation group (PO, n = 30): only exposed blood vessels without clipping. The animals were killed by 4% paraformaldehyde perfusion at 6h, 12h, 24h, 48h, 72h and 96h. TUNEL and HE staining were used to observe the morphological changes and c- Myc in CA1 and CA3 area of different expression and positive neurons, for statistical analysis. Results: 6 hours after cerebral ischemia-reperfusion, c-Myc was expressed in CA1 area of IR group, the intensity of expression increased from 24 to 48 hours, decreased after 72 hours, and weaker in CA3 area than in CA1 area, mainly in cytoplasm. 2HE staining showed that at 72h after ischemia / reperfusion, the edema of CA1 area in IR group was obvious, the number of neurons was reduced, the arrangement was disorganized, the nuclear membrane was unclear, the nucleolus disappeared and the neurons in CA3 area changed slightly. The results of 3TUNEL staining showed that the number of hippocampal CA1 positive cells in IR group was the highest at 24-48 hours after global cerebral ischemia and reperfusion, and the number of hippocampal CA1 positive cells decreased after 72 hours of reperfusion. CONCLUSION: The c-Myc protein is expressed in hippocampus CA1 and CA1 areas after global cerebral ischemia and reperfusion, but the intensity and cell distribution are different.