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Western Blot即蛋白质转染技术是70年代末、80年代初发展起来的一种蛋白质检测新技术。其基本过程是将已经电泳到聚丙烯酰胺凝胶中的蛋白质转移到硝酸纤维素固相膜上,进行蛋白染色或免疫学测定。一、Western Blot Analysis实验原理: 当蛋白质在聚丙烯酰胺凝胶电泳分离后,可出现许多条蛋白质区带。由于各组分分散于凝胶介质中而不能被有效地检测。应用电转移技术,将凝胶中的蛋白质多肽抗原样品,转移到固相载体上,克服了凝胶中检测蛋白条带和相互功能关系中的困难,再通过酶标记或同位素标记免疫测定,即可敏感而简单地检测这些抗原抗体之间的特异性反应。
Western Blot protein transfection technology is a new protein detection technology developed in the late 1970s and early 1980s. The basic process is to transfer proteins that have been electrophoresed to polyacrylamide gels to nitrocellulose solid-phase membranes for protein staining or immunological assays. First, Western Blot Analysis Experimental principle: When the protein in polyacrylamide gel electrophoresis separation, there may be a number of protein bands. The components can not be effectively detected because they are dispersed in the gel medium. The use of electrotransport technology to transfer the protein polypeptide antigen sample in the gel to the solid support overcomes the difficulties in detecting the protein bands and the mutual functional relationships in the gel and then by enzymatic or isotopic labeling immunoassay Sensitive and simple detection of these antigen-specific reaction between antibodies.