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本文建立了一种香菇交配型基因测定的新方法——原生质单核体系本交配到极性标记法.用这一方法不仅成功地分离和鉴定出不同香菇菌株的四种标准交配型基因,还有效地区分出亲本型和重用型两种交配型基因类型。这一技术为深入开展异宗结合食用菌的遗传研究提供了一个重要的工具。
In this paper, we established a new method for the Mating genotype determination of shiitake mushrooms: the protoplasm mononuclear system was mated to the polar marker method. This method not only successfully isolated and identified four standard matings of different mushroom strains, Effectively distinguish between the two types of mating type of parental and reusable genes. This technique provides an important tool for further research on the genetic research of Heterophile combining edible fungi.