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由光棘豆种子无菌苗的胚轴和子叶诱导的愈伤组织建立起细胞悬浮系。由悬浮系分离出单细胞。单细胞在含有1～2mg/L 2,4—D的2/3MS培养基中采用振荡和静止两种方法培养。结果表明。振荡培养愈伤组织再生频率较高,所需时间较短。单细胞再生的愈伤组织转入分化培养基后分化出芽。幼苗转入含有IBA和IAA的1/2MS培养基中生根,成为完整的再生植株。在分化培养基中,只有瘤状愈伤组织能够分化出芽。并且较高浓度的细胞分裂素(6—BA或KT)或6—BA与ZT结合使用对愈伤组织的分化是有利的。
The cell suspension system was established by the embryonic axis and cotyledon-induced callus of Oxya chinensis seeds. Single cells are isolated from the suspension system. Single cells were cultured in 2/3 MS medium containing 1 to 2 mg / L 2,4-D using both shaking and resting methods. The results show. Oscillating cultured callus regeneration frequency is higher, the time required is shorter. Single cell regenerated callus differentiated into shoots after differentiation into shoots. Seedlings were transplanted into 1 / 2MS medium containing IBA and IAA for rooting, becoming a complete regenerated plant. In differentiation medium, only the callus callus can differentiate budding. And the use of higher concentrations of cytokinin (6-BA or KT) or 6-BA in combination with ZT is advantageous for callus differentiation.