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目的克服多聚乙烯亚胺(PEI,polyethlen im ine)/DNA载体对细胞的毒性以及在含血清培养基里对癌细胞基因的转移率低的问题。方法利用具有水溶性、可生物降解的、并带有负电的藻酸盐(alginate)对PEI/DNA载体进行包衣,制备出复合载体,并在体外含50%血清培养基里,与PEI/DNA载体比较对C3癌细胞转染率。结果在含50%血清的培养基里,藻酸盐包衣制备的复合体载体[alginate:DNA,0.15(w/w);PEI:DNA,N:P=10]与PEI/DNA载体相比,对C3癌细胞基因转染率高出10~30倍,而且其表面正电荷数比PEI/DNA载体减少了一半,颗粒较小,并降低对细胞毒性和红血球集聚反应。结论作为新型的藻酸盐包衣制备的复合载体能提高在体外含高浓度血清培养基里对C3癌细胞的转染率,并能减少其对细胞毒性。
Objective To overcome the cytotoxicity of polyethylenimine (PEI) / DNA vector and the low transfer rate of cancer cell genes in serum-containing medium. Methods The PEI / DNA vector was coated with a water-soluble, biodegradable and negatively charged alginate to prepare a complex vector. The vector was incubated with 50% serum in vitro and incubated with PEI / DNA vector comparison of C3 cancer cell transfection efficiency. Results Alginate-coated complex vector [alginate: DNA, 0.15 (w / w); PEI: DNA, N: P = 10] was compared to the PEI / DNA vector in a 50% serum- , Transfection of C3 cancer cells gene rate 10 to 30 times higher, and the surface of the positive charge than the PEI / DNA carrier reduced by half, smaller particles, and reduce the cytotoxicity and erythrocyte agglutination. Conclusion The composite carrier prepared by the novel alginate coating can increase the transfection efficiency of C3 cancer cells in high concentration serum medium in vitro and reduce its cytotoxicity.