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采用DEAE Cellulose、DEAE SephadexA-50、Sephadex G-200三种不同层析柱通过柱层析路径1、2对广东虫草多糖(CGP)进行分离纯化。并利用凝胶渗透色谱法(GPC)测定各柱层析组分的保留时间tR、重均分子量Mw、多分散性PD以及各组分的相对含量,以表征不同柱层析方法所得多糖组分的分子量分布。结果表明:大分子量多糖组分CGP-Ⅰ经过柱层析路径1分离纯化后,分子量分布由宽变窄,分子量略有降低,多糖组分的纯度得到了很好地提高;CGP经过不同柱层析路径中的NaCl梯度洗脱后,得到的都是分子量分布较均匀的小分子多糖。GPC结果显示柱层析路径1对CGP的分离纯化效果优于路径2。
The CGP was separated and purified by column chromatography 1,2 with DEAE Cellulose, DEAE Sephadex A-50 and Sephadex G-200. And the retention time tR, weight average molecular weight Mw, polydispersity PD and the relative content of each component of each column chromatography component were determined by gel permeation chromatography (GPC) to characterize the polysaccharide components obtained by different column chromatography methods The molecular weight distribution. The results showed that the molecular weight distribution of CGP-Ⅰ, a component of large molecular weight polysaccharide, was narrowed and broadened in width after purification by column chromatography. The molecular weight of CGP-Ⅰ decreased slightly and the purity of polysaccharide components increased well. CGP passed through different column Analysis of the path of NaCl gradient elution, are obtained more uniform molecular weight distribution of small molecule polysaccharides. The results of GPC showed that the purification of CGP by column chromatography route 1 was better than that of route 2.