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利用基因组勘测序列(GSS序列)探讨开发南方红豆杉(Taxus wallichiana var.mairei)SSR标记的可行性。从1923条GSS序列中搜寻到184个SSR位点。其中二核苷酸、三核苷酸和四核苷酸重复所占比例分别为88.6%、10.3%和1.1%。不同核苷酸在重复序列中的频率分析表明,a和t所出现的频率远远高于c和g的频率。对所获得的184个SSR位点设计引物,开发出90个候选SSR引物。在所获得的获选标记中选取20对引物在南方红豆杉中进行试验检验,结果19对(95%)SSR引物能够获得清晰稳定的主带,表明GSS序列可以高效地用于开发基因组SSR标记,这些标记将为南方红豆杉的相关研究奠定基础。
The feasibility of developing SSR markers of Taxus wallichiana var. Mairei was explored by using the genome sequence (GSS sequence). 184 SSR loci were found from 1923 GSS sequences. The proportions of dinucleotide, trinucleotide and tetranucleotide repeats were 88.6%, 10.3% and 1.1% respectively. Frequency analysis of different nucleotides in repeated sequences showed that the frequencies at which a and t occur are much higher than the frequencies of c and g. Primers were designed for the 184 SSR loci obtained and 90 candidate SSR primers were developed. Twenty pairs of primers were selected and tested in Taxus chinensis. The results showed that 19 pairs (95%) of SSR primers could obtain a clear and stable main band, indicating that the GSS sequences can be efficiently used for the development of genomic SSR markers , These markers will lay the foundation for the relevant research of Taxus chinensis.