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目的：观察环孢素Ａ是否有诱导白血病细胞凋亡的作用。方法：应用细胞形态学检查、二苯胺法ＤＮＡ片段化定量，ＤＮＡ凝胶电泳及流式细胞术等方法观察细胞凋亡。结果：ＣｓＡ５０ｍｇ／Ｌ作用ＨＬ－６０细胞４ｈ，ＤＮＡ片段率显著增高，达（２８．２±５．８）％，而对照组仅为（１２．５±１．７）％（Ｐ＜０．０１，ｎ＝１０）。光镜及电镜检查见细胞核固缩，碎裂，有凋亡小体形成。ＤＮＡ电泳显示典型的ＤＮＡｌａｄｄｅｒ。流式细胞术检测发现ＣｓＡ５０ｍｇ／Ｌ作用ＨＬ－６０细胞６ｈ凋亡细胞率为４９．７％，而空白组仅为９．１％。ＣｓＡ诱导ＨＬ－６０细胞ＤＮＡ片段化呈剂量和时间依赖性。结论：ＣｓＡ在体外能诱导ＨＬ－６０细胞凋亡。
Objective: To observe whether cyclosporine A can induce apoptosis of leukemia cells. Methods: Apoptosis was observed by cell morphological examination, quantitative DNA fragmentation by diphenylamine, DNA gel electrophoresis and flow cytometry. RESULTS: After treated with CsA50mg/L for HL-60 cells for 4h, the DNA fragment rate was significantly increased (28.2±5.8)% compared with (12.5±1.7)% in the control group (P<0. 01, n=10). Light microscopy and electron microscopy showed nuclei pyknosis, fragmentation, and formation of apoptotic bodies. DNA electrophoresis shows a typical DNA ladder. Flow cytometry showed that the apoptotic cell rate of CsA50mg/L HL-60 cells at 6h was 49.7%, while that of the blank group was only 9.1%. CsA induced DNA fragmentation of HL-60 cells in a dose- and time-dependent manner. Conclusion: CsA can induce apoptosis of HL-60 cells in vitro.