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[目的]从受铬污染的土壤中分离抗铬微生物。[方法]利用微生物分离纯化技术,在培养基中加入不同浓度的Cr6+,经过不断的分离和驯化筛选耐铬真菌,根据其形态特征和菌落特征进行初步鉴定,并对其生物学特性进行了初步研究,包括生长曲线的测定、温度、pH值和渗透压对其生长的影响。[结果]从玉林市周边地区受重金属污染较严重的10处土壤中分离筛选到1株在Cr(VI)浓度为1000mg/L时仍生长良好的抗铬真菌,根据其形态特征和菌落特征初步鉴定该菌为酵母菌,该菌在15～37℃的温度范围内生长良好,最适温度为28℃;pH在4～10时,菌株均能生长良好,其中最适生长pH为7.2;在NaCl浓度为0.5%～5%时,均能生长良好。通过抗性试验,发现该菌除了对铬具有较高的抗性外,对其它的重金属Pb、Cu、Fe和复合重金属Pb+Cu、Cu+Fe、Pb+Fe、Pb+Cu+Fe也具有抗性能力。[结论]该试验筛选到的抗铬真菌对自然环境具有较好的适应性,同时该菌对其他重金属也具有抗性。
[Objective] To separate chrome-resistant microorganisms from chromium contaminated soil. [Method] Microbial isolation and purification technology was used to add different concentrations of Cr6 + to the culture medium. Chromium-resistant fungi were isolated and acclimated continuously, and their biological characteristics were preliminarily identified according to their morphological and colony characteristics Studies, including the determination of growth curves, the effects of temperature, pH and osmotic pressure on their growth. [Result] One chrysanthemum fungus which grew well at Cr (VI) concentration of 1000 mg / L was isolated and screened from 10 soils contaminated by heavy metals in the surrounding area of Yulin City. According to its morphological characteristics and colony characteristics The strain was identified as yeast. The strain grew well in the temperature range of 15 ~ 37 ℃ and the optimal temperature was 28 ℃. The strain grew well at pH 4 ~ 10, and the optimum growth pH was 7.2. NaCl concentration of 0.5% to 5%, all can grow well. Through the resistance test, it was found that the fungi had high resistance to chromium, as well as other heavy metals Pb, Cu, Fe and complex heavy metals Pb + Cu, Cu + Fe, Pb + Fe and Pb + Cu + Fe Resistance ability. [Conclusion] The anti-chromium fungi screened by this test had good adaptability to the natural environment, and the strain was also resistant to other heavy metals.