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目的制备抗黑色素瘤相关抗原(MAGE)抗体偶联的载金纳米棒靶向纳米分子探针,探讨其对体外恶性黑色素瘤细胞的靶向性,观察其体外光声成像效果。方法采用双乳化法制备包裹金纳米棒和液态氟碳的聚乳酸-羟基乙酸共聚物(PLGA)纳米粒,碳二亚胺法连接靶向MAGE的单克隆抗体,制备高分子多功能靶向PLGA纳米分子探针,检测其一般物理特性、MAGE抗体与纳米粒的连接情况及其体外寻靶能力,并观察体外光声成像效果。结果成功制备的靶向载金纳米棒多功能纳米分子探针平均粒径(336.40±27.46)nm,平均Zeta电位(-4.34±4.9)m V。MAGE抗体与纳米粒有效连接。体外寻靶能力实验显示黑色素瘤B16细胞株周围有大量靶向纳米探针环绕;光声成像显示随金纳米粒含量增加,其光声信号逐渐增强。结论 MAGE抗体偶联金纳米棒纳米分子探针制备成功,对体外恶性黑色素瘤细胞具有较好的生物活性,细胞靶向性较好,且体外光声成像有明显增强信号,可作为光声成像造影剂,进一步行肿瘤靶向光热治疗。
OBJECTIVE: To prepare targeted gold nanoparticle probes conjugated to anti-melanoma-associated antigen (MAGE) antibody and investigate its targeting to malignant melanoma cells in vitro. Methods Poly (lactic-co-glycolic acid) (PLGA) nanoparticles coated with gold nanorods and liquid fluorocarbons were prepared by double emulsification. Monoclonal antibodies targeting MAGE were linked by carbodiimide method to prepare PLGA Nano-molecular probe to detect its general physical properties, MAGE antibody and nanoparticle connection and in vitro targeting ability, and observe the effect of photoacoustic imaging in vitro. Results The average size of the prepared nanoparticle probe with gold nanoparticles was 336.40 ± 27.46 nm and the average Zeta potential was -4.34 ± 4.9 mV. MAGE antibody is effectively linked to the nanoparticle. In vitro targeting ability experiments showed that a large number of targeted nano-probes surround the melanoma B16 cell line. Photoacoustic imaging showed that the photoacoustic signal gradually increased with the increase of the gold nanoparticle content. Conclusions The MAGE-conjugated gold nanorods probe has been successfully prepared and has good biological activity against malignant melanoma cells in vitro and good cell targeting. In addition, the photoacoustic imaging of MAGE antibody significantly enhances the signal and can be used as a photoacoustic imaging Contrast agent, further tumor targeting photothermal therapy.