,Class I phosphatidylinositol 3-kinase inhibitor LY294002 activates autophagy and induces apoptosis

• 作 者: Chungen Xing Baosong Zhu Huihui Liu Huihua Yao Lifeng Zhang
• 机 构: Department of General Surgery, The Second Affiliated Hospital, Soochow University, Suzhou 215004, Ch
• 出 处: 生物化学与生物物理学报（英文版）
• 发表日期: 2008年3期
• 关键词: PI3K LY294002 autophagy apoptosis

We aimed to study the effects of LY294002, an inhibitor of classIphosphatidylinositol 3-kinase (PDK), on proliferation,apoptosis, and autophagy in gastric cancer cell line SGC7901.In this study, we showed that LY294002 inhibited the viability of gastric cancer SGC7901 cells.We also showed that LY294002 increased the expression of microtubule-associated protein 1 light chain 3(LC3),and increased monodansylcadaverine(MDC)-labeled vesicles.LY294002 activated autophagy by activating p53 and caspase-3,and induced apoptosis by up-regulating p53 and p53-up-regulated modulator of apoptosis(PUMA).Therefore,LY294002 might induce cytotoxicity in SGC7901 cells through activation of p53 and the downstream point PUMA.These findings suggest that inhibition of the class I PI3K signaling pathway is a potential strategy for managing gastric cancers.