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目的提出一种大鼠尺骨运动疲劳损伤模型的在体建立方法,为后续骨组织运动疲劳损伤特点及修复重建的实验研究提供平台和依据。方法选择3个月龄雌性Wistar大鼠36只,体质量约250 g。利用前期文献查阅、有限元计算分析和大鼠离体尺骨疲劳力学性能测定结果,对大鼠尺骨组织施加在体疲劳加载,建立在体运动疲劳损伤模型。从微观-宏观多个层面检测疲劳加载后骨组织细胞的响应、显微损伤情况及骨组织力学性能的变化,验证模型的有效性。结果按照确定的运动疲劳加载条件(1.5 Hz,3 000με,8 000次/2天),经过1周在体疲劳加载,大鼠尺骨割线模量和循环能量耗散值下降显著,尺骨中段截面制片中观察到显微裂纹,空白骨细胞陷窝的比例(40.96%±4.88%)显著升高,破骨细胞活性显著增强(约为对照组的1.45倍),成骨细胞活性相对减弱(约为对照组的87%)。从多个层面证明骨组织疲劳和显微损伤的出现,进而验证了大鼠尺骨在体运动疲劳损伤模型的成功建立。结论成功建立并验证了一种大鼠尺骨在体运动疲劳损伤模型,可以为军队士兵或中长跑运动员运动疲劳损伤预防与治疗的相关研究提供动物模型和理论支持。
OBJECTIVE: To establish a method to establish a model of rat ulnar motion fatigue injury in order to provide a platform and basis for the follow-up study of the characteristics of the dynamic fatigue damage of bone tissue and its reconstruction. Methods Thirty-six three-month-old female Wistar rats were selected and their body weight was about 250 g. Based on the results of previous literature review, finite element analysis and fatigue analysis of rat ulnar ulnar fatigue, the rats’ ulnar tissues were subjected to body fatigue loading to establish a model of physical fatigue injury. The response of bone cells, the micro-damage and the changes of mechanical properties of bone tissue after fatigue loading were examined at the micro-macro level to verify the effectiveness of the model. Results After fatigue loading for 1 week, the secant modulus and circulating energy dissipation decreased significantly in accordance with the determined conditions of fatigue loading (1.5 Hz, 3 000 με, 8 000/2 days) The microcracks were observed in the films with a significant increase in the ratio of osteoblasts (40.96% ± 4.88%) and osteoclast activity (about 1.45 times that of the control group), and a relative decrease in osteoblast activity About 87% of the control group). Bone tissue fatigue and micro-damage were demonstrated at various levels, and the successful establishment of a rat model of sports-induced fatigue in the ulna was verified. Conclusion A rat model of ulnar fatigue injury in vivo is successfully established and validated, which can provide animal models and theoretical support for the research on the prevention and treatment of sports fatigue injuries in soldiers or middle-long distance runners.