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一、接种后出现死种的原因及处理香菇接种后出现死种原因:①培养基配制拖延时间长,致使pH值升高,出现腐败。香菇菌丝pH5—6最适,7受到影响,8无法生长;②培养基灭菌不彻底,导致发臭。菌种“闷死”。一般料袋进灶要求在5小时内要上100℃,上温后保持14小时,中途不停火,不降温。如果进灶时间拖长,上温100℃后,中途停火降温,袋料容易发酵变臭,灭菌彻底的袋料会发出香味;③接种时袋内温度超过32℃以上,使菌种烫死;④接种块入穴太浅,使种与料不能吻合,菌种悬浮无法定植料内而逐渐干枯,这叫“干死”;⑤料质含水量偏多,超过60％,接种后菌种干湿反差过大,菌种被“浸死”。上述情况处理办法:①对穴内无菌丝定植,又无杂菌污染的,补接菌种;对穴内已污染杂菌
First, the reasons for dead after inoculation and the treatment of dead mushrooms after inoculation reasons: ① medium preparation delay for a long time, resulting in increased pH, there corruption. Mushroom mycelium pH5-6 optimum, 7 affected, 8 can not grow; ② medium sterilization is not complete, resulting in smelly. Strain “suffocated.” General feeding requirements into the bag 100 hours in 5 hours to keep the temperature after 14 hours, half-way non-stop fire, do not cool down. If prolonged into the stove for a long time, the temperature of 100 ℃, the middle of the cease-fire cooling, the bag easily fermented smelly, sterilization will be issued a sack of bag full of flavor; ③ inoculation bag temperature exceeds 32 ℃ above, ; ④ vaccination block too shallow, so that the species can not match with the material, the bacteria can not be suspended within the suspended material gradually dry, called “dry death”; ⑤ material moisture content more than 60%, after inoculation strain dry Wet contrast is too large, bacteria were “drowned.” The above approach to deal with: ① on the hole without mycelium colonization, and no bacterial contamination, and then add bacteria; the cavity has been contaminated bacteria