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一、材料与方法: 1、标准毒株:甲_3/粤防77-38、甲_3/京科79-2、甲_1/津防77-78、乙/湘防72-4由省防疫站供给。 2、标本采取:咽拭标本采取临床症状典型和类似流感患者的咽拭子。双份血清系上述患者急性期(发病2～3天)、恢复期(2～3周后)耳垂血。正常人群血清系数收集医院肝功能正常血清。 3、方法:病毒分离按常规接种9～11日令鸡胚尿囊和羊膜腔。以血凝抑制试验鉴定病毒;交互血凝抑制试验作抗原分析;半加敏微
First, the material and methods: 1, the standard strains: A _3 / Guangdong defensive 77-38, A _3 / Jingke 79-2, A _1 / Tianjin anti-77-78, B / Hunan anti-72-4 by Provincial epidemic prevention station supply. 2, specimens taken: pharyngeal swab specimens to take the clinical symptoms of throat swabs in patients with typical and flu-like. Two copies of serum in patients with acute phase (onset 2 to 3 days), recovery (2 to 3 weeks later) earlobe blood. Normal population Serum coefficient Collection of normal liver function in hospital serum. 3, Methods: Virus isolation according to conventional vaccination 9 to 11 so that chicken embryo allantoic and amniotic cavity. Hemagglutination inhibition test identified virus; interactive hemagglutination inhibition test for antigen analysis; semi-sensitive