论文部分内容阅读
目的 研究在体外培养条件下血管内皮细胞条件培养基 (ECCM)、内皮素 - 1(ET- 1)、内皮素转化酶抑制剂 phos-phoramidon对血管平滑肌细胞 (SMC)增殖的影响 ,同时研究了血管平滑肌细胞条件培养基 (SMCCM)对血管内皮细胞(EC)增殖方面的影响 .方法 EC和 SMC均来源于兔主动脉 ,在获得了 EC和 SMC的条件培养基 (CM)后 ,分别用两者以及 ET- 1进行实验 ,细胞的增殖率通过 3H掺入法进行测定 .结果 ECCM和 ET- 1可明显促进 SMC的增殖 ,并呈现剂量依赖性 .其最大效应分别为 (190± 11) % (10 0 % ECCM)和(16 6± 9) % (10 0 ng· L- 1 ET- 1) .而 phosphoramidon存在条件下的 ECCM使其分裂作用降低了 (33± 2 ) % .SMCCM抑制 EC的增殖 ,这种抑制作用并非剂量依赖性 ,其最大的抑制效应为基本水平的 (78± 3) % .结论 ECCM和 ET- 1可促进 SMC的增殖作用 .phosphoram idon可明显地抑制 ECCM对 SMC的增殖作用 .同时 SMCCM对 EC的增殖起抑制作用
Objective To investigate the effects of endothelin-1 (ET-1) and endothelin-converting enzyme inhibitor phos-phoramidon on the proliferation of vascular smooth muscle cells (SMCs) in vitro under culture conditions. The effects of vascular smooth muscle cell conditioned medium (SMCCM) on the proliferation of vascular endothelial cells (EC) .Methods Both EC and SMC were derived from the aorta of rabbits. After obtaining EC and SMC conditioned medium (CM) And ET-1, the cell proliferation rate was determined by 3H incorporation method.Results ECCM and ET-1 could significantly promote the proliferation of SMC in a dose-dependent manner.The maximum effects were (190 ± 11)% (10 0% ECCM) and (16 6 ± 9)% (10 0 ng · L -1 ET-1), while the ECCM in the presence of phosphoramidon reduced the cell division by 33 ± 2% (78 ± 3)% .Conclusion ECCM and ET-1 can promote the proliferation of SMC.phosphoram idon can significantly inhibit the ECCM on SMC Proliferation of SMCCM while EC increased Disincentive