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本研究用OK系列单克隆抗体对肝包虫患者肝组织的B、T淋巴细胞及其亚群进行免疫组化染色,且对其细胞密度、比值进行定量分析。发现肝包虫患者肝组织(距病灶>5cm),外囊及毗邻肝组织中CD_3~+、CD_(19)~+、CD_4~+及CD_8~+细胞密度均较对照组显著增高(P<0.01),外囊及毗邻肝组织CD_4~+/CD_8~+细胞的比值较肝包虫肝组织及对照组均有显著降低(P<0.01)。肝包虫肝组织内CD_(19)~+细胞与CD_3~+细胞密度间无显著性差异(P>0.1)。在肝实质区的灶状坏死区内,CD_8~+细胞占优势。实验结果表明,在肝包虫感染时体液免疫及细胞免疫均增强,局部组织反应中CD_8~+细胞占优势提示细胞免疫以抑制性免疫为主,且此抑制性免疫反应有梯度变化,距包虫囊越近则免疫抑制越强,CD_4~+细胞与CD_8~+细胞密度改变可能与肝组织纤维化有关。包虫病时体液免疫与细胞免疫为两个独立的免疫系统,无相关关系。本文研究结果可为包虫病患者化疗、预后提供科学依据。
In this study, immunohistochemical staining of B and T lymphocytes and their subpopulations in hepatic tissue of patients with hepatic hydatid were performed by OK monoclonal antibody, and their cell density and ratio were quantitatively analyzed. It was found that the liver tissue of hepatic hydatid (> 5cm away from the lesion), the density of CD_3 ~ +, CD_ (19) ~ +, CD_4 ~ + and CD_8 ~ + in the outer capsule and adjacent liver tissue were significantly higher than those in the control group 0.01). The ratio of CD_4 ~ + / CD_8 ~ + cells in the outer capsule and the adjacent liver tissue was significantly lower than that in the hepatic hydatid liver tissue and the control group (P <0.01). Liver liver hydatid liver CD_ (19) ~ + cells and CD_3 ~ + cell density was no significant difference (P> 0.1). CD_8 ~ + cells predominate in the focal necrotic area of the parenchyma of the liver. The experimental results showed that humoral immunity and cellular immunity were all enhanced during hepatic hydatid infection, and predominance of CD_8 ~ + cells in local tissue reaction suggested that cellular immunity was dominated by inhibitory immunity, and the inhibitory immune response had a gradient change. The closer the worm capsule, the stronger the immunosuppression, CD_4 ~ + cells and CD_8 ~ + cell density changes may be related to liver fibrosis. Echinococcosis humoral immunity and cellular immunity as two separate immune systems, no correlation. The results of this study may provide a scientific basis for chemotherapy and prognosis of patients with hydatid disease.