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目的:研究小叶莲的化学成分指纹图谱,评价小叶莲商品药材质量,并比较桃儿七不同药用部分之间的主要成分差异,为临床安全、合理应用提供依据。方法:采用小叶莲HPLC指纹图谱方法,测定了12份小叶莲的指纹图谱,确定了20个色谱峰作为共有峰,并指认了其中12个色谱峰;运用该方法比较了桃儿七根及根茎与小叶莲指纹图谱的差异,通过相似度评价和聚类分析对图谱数据进行分析。结果:本研究首次报道了小叶莲中4个黄酮类成分;首次指认了小叶莲指纹图谱中11个色谱峰,包括9个黄酮和2个木脂素类成分;桃儿七不同药用部分化学成分存在较明显的差异,小叶莲主要含异戊烯基黄酮类成分,桃儿七根和根茎主要含鬼臼毒素和简单取代的黄酮及其苷类成分。结论:该指纹图谱方法可以作为评价比较小叶莲、桃儿七根及根茎中黄酮类和木脂素类成分的相对含量及其质量的依据。同时,为小叶莲、桃儿七根及根茎不同药用部分的药效学差异研究提供参考。
OBJECTIVE: To study the fingerprint of chemical constituents of Xiaoyunshan, evaluate the quality of Xiaoyulian medicinal materials, and to compare the difference of the main components among the seven different medicinal parts of Haemonchum for clinical safe and reasonable application. Methods: The fingerprinting of 12 small lotus seeds was determined by HPLC fingerprinting method of Xiao Ye Lin. 20 peaks were identified as common peaks and 12 peaks were identified. Seven peaches and rhizomes were compared by this method And Lotus leaf lotus fingerprinting differences, through the similarity evaluation and cluster analysis of the data analysis. Results: Four flavonoids were identified for the first time in this study. Eleven chromatographic peaks were identified in the fingerprints of C. indicum for the first time, including nine flavonoids and two lignan components. Seven different medicinal herbs There are obvious differences in the components, the main content of the leaf lotus isopentenyl flavonoids, peach seven and rhizome containing podophyllotoxin and simple replacement of flavonoids and glycosides. Conclusion: This fingerprinting method can be used as a basis for evaluating the relative content and quality of flavonoids and lignans in seven and root of peach. At the same time, it provided references for the pharmacodynamics differences of different medicinal parts of Xiaolian lotus, peach seven roots and rhizomes.