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以番茄毛粉802种子为材料,通过对比实验,测定分析各处理种子的萌发率及第4天的平均根长、萌发指数、活力指数,以及相对电导率(REC)、丙二醛(MDA)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)含量的变化,以探讨NO对番茄种子吸胀冷害的抵抗作用及其机理.结果显示:(1)外源NO可显著提高番茄种子经12 h吸胀冷害处理后的萌发率、平均根长、萌发指数和活力指数,并显著降低吸胀冷害下REC和MDA含量,同时显著提高SOD和CAT的含量.(2)NO所提高的吸胀冷害处理后种子的SOD和CAT活性不能被RNA合成抑制剂放线菌素D和蛋白质合成抑制剂环己酰亚胺抑制.结果表明,NO可提高番茄种子抵抗吸胀冷害的能力,而且与NO激活了抗氧化系统有关,但NO不是通过促进抗氧化酶的合成来提高其活性.
The germination rate and the average root length, germination index, vitality index, relative conductivity (REC), malondialdehyde (MDA) , Superoxide dismutase (SOD) and catalase (CAT) in tomato seeds were measured to explore the resistance and mechanism of NO on the chilling injury of tomato seeds.The results showed that: (1) Exogenous NO significantly increased the content of tomato The germination rate, average root length, germination index and vigor index of seeds after 12 h of cold acclimation treatment significantly reduced the content of REC and MDA, and significantly increased the content of SOD and CAT. (2) The increase of NO The SOD and CAT activities of seeds after inbred chilling treatment could not be inhibited by actinomycin D and cycloheximide which is the inhibitor of protein synthesis.The results showed that NO could enhance the ability of tomato seed to resist the chilling injury, And NO activated antioxidant system, but NO is not through the promotion of antioxidant enzyme synthesis to increase its activity.