论文部分内容阅读
对‘新陆早17’和‘中棉49’两个棉花(Gossypium hirsutumL.)品种在氧化剂甲基紫精(MV)和NaCl胁迫下的生理应答进行了研究,以期阐明棉花抗氧化能力与耐盐性的关系。研究结果表明,在40μmol·L-1MV胁迫3d,‘新陆早17’的丙二醛(MDA)和活性氧含量比‘中棉49’低,而抗坏血酸(AsA)、超氧化物岐化酶(SOD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性则高于‘中棉49’,表明‘新陆早17’抗氧化能力更强。在200mmol·L-1NaCl胁迫25d,‘新陆早17’的株高和叶绿素降低量、根部Na+含量、相对电导率都低于‘中棉49’,说明‘新陆早17’耐盐更强。盐胁迫下‘新陆早17’的抗氧化酶活性上升而‘中棉49’却降低,表明‘新陆早17’通过上调SOD、CAT和APX活性来提高其耐盐性。结合棉花两品种的抗氧化和耐盐性差异,说明棉花抗氧化能力的高低能够影响品种的耐盐性。
The physiological responses of Gossypium hirsutum L. cultivars (Xinluza 17 and Zhongmian 49) under the oxidative stress of methyl viologen (MV) and NaCl were studied in order to clarify the antioxidant capacity and tolerance of cotton Salt relations. The results showed that the content of malondialdehyde (MDA) and reactive oxygen species of ’Xinluzao17’ was lower than that of ’Zhongmian49’ at 40μmol·L-1MV for 3 days, while the contents of AsA and superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) were higher than that of ’Zhongmian 49’, indicating that ’Xinluxao 17’ was more resistant to oxidation. Under the stress of 200 mmol·L-1 NaCl for 25 days, the plant height, the chlorophyll content, the Na + content and the relative conductivity of ’Xinluxao17’ were lower than those of ’Zhongmian49’ . Under salt stress, the activity of antioxidant enzymes of ’Xinluxao17’ increased and ’Zhongmian49’ decreased, which indicated that Xinluzao17 increased its salt tolerance by up-regulating the activity of SOD, CAT and APX. Combining the difference of antioxidant and salt tolerance between two varieties of cotton, it shows that the antioxidant ability of cotton can affect the salt tolerance of the varieties.