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目的 为确定溪黄草规范化种植基地及药用部位、制定相关标准操作规程(SOP)并实施推广应用,提供基础研究资料。方法 采用RP-HPLC测定不同种植基地、不同植株部位溪黄草中2α-羟基熊果酸的含量。色谱条件:Kromasil RP-C18 色谱柱(4.6 mm×250 mm,5 μm);乙腈-0.05％三氟乙酸溶液(70.5:29.5)为流动相;流速0.8 mL·mim-1;柱温25℃;检测波长210 nm。结果 清远种植基地溪黄草中2α-羟基熊果酸的含量高于饶平;清远、饶平种植基地溪黄草不同植株部位中2α-羟基熊果酸含量均以叶中为高。结论 应结合其他研究结果进一步考察确立溪黄草规范化种植基地;建议以溪黄草的叶入药为佳。
Objectives To determine the standardized cultivation bases and medicinal parts of Stipa, to formulate relevant SOPs and to implement the application and provide basic research data. Methods RP-HPLC was used to determine the content of 2α-hydroxyl ursolic acid in different planting sites and different plant parts. Chromatographic conditions: Kromasil RP-C18 column (4.6 mm×250 mm, 5 μm); acetonitrile-0.05% trifluoroacetic acid solution (70.5:29.5) as the mobile phase; flow rate 0.8 mL·mim-1; column temperature 25°C; Detection wavelength 210 nm. Results The content of 2α-hydroxyl ursolic acid in Qinghuangcao plant was higher than that in Raoping; the contents of 2α-hydroxyl ursolic acid in different parts of the plant were all higher in the middle leaves than in the Qingyuan and Raoping plantations. Conclusion It is necessary to further inspect the establishment of standardized planting bases of Xihuangcao in combination with other research results; it is recommended to use the leaves of Phellodendron chinense as medicine.