Research on the SFE-CO_2 Extraction of Essential Oils from P.massoniana Lamb Needles and Their Chemi

• 作 者: LI Hai-long HE Yu-you HU Shui-bao
• 机 构: Zhejiang Yalin Biotech Co.,Ltd.,
• 出 处: Medicinal Plant
• 发表日期: 2012年09期
• 关键词: supercritical preliminary optimize chosen distillation contained matching separa

[Objective] To optimize the supercritical CO2 process parameters of essential oils from the needles of Pinus massoniana Lamb and to identify the chemical constituents of essential oils.[Method] Taking the yield of essential oils as an index,the proper factors and levels were chosen through the preliminary test,and the interaction between factors was observed by orthogonal test.Moreover,GC-MS analysis was used to identify the chemical constituents of essential oils.[Result] The optimized technology of supercritical CO2 extraction of essential oils from P.massoniana needles was extraction pressure 18 MPa,extraction temperature 40 ℃,CO2 flow 25 L/h,and pressure of separation kettle 8 MPa.The yield of essential oils was as much as 0.163% using the optimized process parameters.The main chemical constituents of essential oils were α-pinene,β-pinene,and carypohyllene with content more than 50%.[Conclusion] This method can screen out the supercritical CO2 process parameters of essential oils from P.massoniana needles.It can provide basis for development and utilization of essential oils from P.massoniana needles.

[Objective] To optimize the supercritical CO2 process parameters of essential oils from the needles of Pinus massoniana Lamb and to identify the chemical constituents of essential oils. [Method] Taking the yield of essential oils as an index, the proper factors and levels were chosen through the preliminary test, and the interaction between factors was observed by orthogonal test. Moreover, GC-MS analysis was used to identify the chemical constituents of essential oils. [Result] The optimized technology of supercritical CO2 extraction of essential oils from P. massoniana needles was extraction pressure 18 MPa, extraction temperature 40 ° C, CO2 flow 25 L / h, and pressure of separation kettle 8 MPa. The yield of essential oils was as much as 0.163% using the optimized process parameters. The main chemical constituents of essential oils were α-pinene, β-pinene, and carypohyllene with content more than 50%. [Conclusion] This method can screen out the supercritical CO2 process parameters of essential oi ls from P. massoniana needles. It can provide basis for development and utilization of essential oils from P. massoniana needles.