论文部分内容阅读
目的:探讨女性下生殖道炎症患者发酵支原体(Mf)、穿通支原体(Mpe)、梨支原体(Mpi)、解脲脲原体(Uu)以及人型支原体(Mh)的感染情况,为临床诊治提供依据。方法:病例组(280例,其中宫颈炎120例,阴道炎100例,宫颈炎合并阴道炎60例)和对照组(280例)的宫颈或阴道分泌物,分别接种于改良SP-4和Uu、Mh培养基进行分离培养。培养阳性菌株采用生化反应试验、套式聚合酶链反应(nPCR)和核酸序列测定予以鉴定。结果:①病例组分离出阳性菌株144株(占51.43%),其中Mf8株(占2.86%),Mpe7株(占2.50%),Uu75株(占26.79%),Mh54株(占19.29%),未分离出Mpi;对照组仅分离出5株Uu(占1.79%)。系列鉴定结果与培养结果一致。②病例组与对照组在发酵支原体和穿通支原体的分离率上有统计学意义(P<0.01)。③宫颈炎组与阴道炎组间支原体的总分离率差异有显著性(P<0.01)。结论:女性下生殖道炎症与五种支原体感染有关;妇科炎症患者存在AIDS相关支原体感染,在临床诊治中应给予足够关注。
Objective: To investigate the infection of Mycoplasma fermentum (Mf), Mpe, Mpi, Uu and Mh in women with genital tract inflammation in women and to provide clinical diagnosis and treatment in accordance with. Methods: Cervical or vaginal secretions of patients (280 cases, including 120 cases of cervicitis, 100 cases of vaginitis, 60 cases of cervicitis with vaginitis) and control group (280 cases) were inoculated into modified SP-4 and Uu , Mh medium for isolation and culture. Cultured positive strains biochemical tests, nested polymerase chain reaction (nPCR) and nucleic acid sequence determination to be identified. Results: ①In the case group, 144 strains (51.43%) were positive, of which Mf8 (2.86%), Mpe7 (2.50%), Uu75 (26.79%), Mh54 (19.29%), Mpi was not isolated; only 5 Uu were isolated from the control group (1.79%). Series identification results and culture results. ② The case group and the control group in the fermentation of Mycoplasma and Mycoplasma penetration rate was statistically significant (P <0.01). ③ cervicitis group and vaginitis group mycoplasma total separation rate difference was significant (P <0.01). CONCLUSIONS: The lower genital tract inflammation in women is related to five mycoplasma infections. There is AIDS-related mycoplasma infection in gynecological inflammation patients, and enough attention should be paid in clinical diagnosis and treatment.