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目的 了解正常口腔黏膜,癌前病变及癌组织中内皮素I(ET-I)表达特点及其变化规律。方法采用免疫组化和图象分析技术,对人正常口腔黏膜(NOR)10例、口腔黏膜下纤维化(OSF)10例、鳞状细胞癌(SCC)15例上皮细胞的ET-I含量进行图像分析。结果 ①ET-I在OSF、OLK、SCC组织中的表达增强,阳性物质主要位于上皮棘细胞、基质细胞的胞浆胞膜上,且ET-I含量显著高于正常对照组(p<0.01)。②OLK、SCC上皮细胞ET-I含量呈显著增加趋势(p<0.05)。③OSF上皮细胞ET-I含量显著高于OLK(p<0.05),与SCC相比无显著性差异(p>0.05)。结论 ET-I含量在口腔癌前病变至癌变过程中可能存在一种量变关系,OSF中ET-I过量表达可能提示其上皮细胞的癌变潜能。
Objective To investigate the expression of Endothelin-1 (ET-I) in normal oral mucosa, precancerous lesions and cancerous tissues and their changes. Methods Immunohistochemistry and image analysis were used to detect ET-I levels in 10 cases of normal human oral mucosa (NOR), 10 cases of oral submucous fibrosis (OSF) and 15 cases of squamous cell carcinoma (SCC) Image analysis. Results ① The expression of ET-I in OSF, OLK and SCC tissues was enhanced. The positive substances were mainly located in the cytoplasm of epithelial spine cells and stromal cells, and ET-I content was significantly higher than that of the normal control group (p <0.01). ② The content of ET-I in OLK and SCC epithelial cells increased significantly (p <0.05). ③ The content of ET-I in epithelial cells of OSF was significantly higher than that of OLK (p <0.05), but no significant difference compared with SCC (p> 0.05). Conclusion There may be a quantitative relationship between the content of ET-I and carcinogenesis in oral precancerous lesions. Over-expression of ET-I in OSF may indicate the carcinogenic potential of epithelial cells.