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目的观察转化生长因子－β１（ＴＧＦβ１）对大鼠肺成纤维细胞Ⅰ、Ⅲ型前胶原和ＴＧＦβ表达的影响。方法应用Ｎｏｒｔｈｅｒｎ印迹杂交和免疫细胞化学法。结果经ＴＧＦβ１作用后，肺成纤维细胞Ⅰ、Ⅲ型前胶原ｍＲＮＡ及其蛋白的表达增强，也可见ＴＧＦβ的ｍＲＮＡ杂交信号和其蛋白的阳性反应。结论ＴＧＦβ１能够促进大鼠肺成纤维细胞合成Ⅰ、Ⅲ型前胶原；ＴＧＦβ能促进肺成纤维细胞自身合成ＴＧＦβ。认为肺成纤维细胞自分泌ＴＧＦβ是肺纤维化进行性进展的重要原因之一。
Objective To observe the effect of transforming growth factor-β1 (TGF-β1) on the expression of type Ⅰ, Ⅲ procollagen and TGFβ in rat lung fibroblasts. Methods Northern blotting and immunocytochemistry were used. Results TGF-β1 effect, lung fibroblasts Ⅰ, Ⅲ procollagen mRNA and its protein expression increased, also visible TGF β mRNA hybridization signal and its protein positive reaction. Conclusion TGF β1 can promote rat lung fibroblasts synthesis type Ⅰ, Ⅲ procollagen; TGF β can promote lung fibroblasts synthesize TGF β. It is considered that fibroblast autocrine TGF β is one of the important reasons for the progressive progress of pulmonary fibrosis.