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提出了一种基于信号放大技术的流动注射化学发光免疫传感器,用于联合检测人血清中甲胎蛋白(AFP)和癌胚抗原(CEA)的含量.该传感器首先用表面接枝羧基的氧化石墨烯来吸附固定辣根过氧化物酶(HRP)标记的二抗,制得信号放大型免疫探针.然后用合成的磁性介孔材料Fe3O4/SiO2来吸附固定AFP和CEA的单克隆抗体,用磁铁将其吸附在两个检测池内壁.当待检样品和信号放大型免疫探针陆续注射进入相应检测池,发生夹心式免疫反应,形成的免疫复合物被磁条吸附在检测池内壁.随后加入鲁米诺和H2O2发光底物,与酶标记物发生酶促增强的化学发光反应,并通过控制设在两个管路上的开关来控制鲁米诺和H2O2的流通,相继检测两个检测池中的化学发光信号,从而实现两种待测物的同时检测.
A flow injection chemiluminescence immunoassay based on signal amplification technology was proposed for the combined detection of AFP and CEA in human serum.The sensor was first coated with graphene oxide Aldehyde to immobilize HRP-labeled secondary antibody to obtain a signal-amplification type immunoprobe, and then the synthetic magnetic mesoporous material Fe3O4 / SiO2 was used to adsorb and immobilize the monoclonal antibody of AFP and CEA The magnet will be adsorbed on the inner wall of the two test cells.When the sample to be seized and amplified signal probe into the corresponding test after another pool, a sandwich-type immune reaction occurs, the immune complexes formed by the magnetic stripe adsorption in the test cell wall. Luminol and H2O2 luminescent substrates were added, enzymatically enhanced chemiluminescence reactions with enzyme labels, and the flow of luminol and H2O2 were controlled by controlling the switches located on both lines, one after the other, two test cells In the chemiluminescence signal in order to achieve the simultaneous detection of two analytes.