论文部分内容阅读
目的对比观察雄激素依赖前列腺癌细胞(LNCaP)与雄激素非依赖前列腺癌细胞(LNCaP-AI)中microRNA的表达差异,探讨前列腺癌激素依赖向非依赖转化的转录后调控机制。方法利用Agilent基因芯片检测LNCaP及LNCaP-AI细胞microRNA的表达,用RT-PCR方法对其中6个microRNA进行验证,并对差异表达的microRNA所调控靶基因功能进行生物信息学分析。结果基因芯片检测发现:与LNCaP相比,LNCaP-AI细胞有27个microRNA表达降低,11个microRNA表达升高。对其中6个microRNA进行RT-PCR验证,结果与基因芯片结果一致。通过检索http://www.mir-base.org/针对microRNA调控的靶基因,并参考已知的与雄激素非依赖相关基因的功能,发现LNCaP细胞转化为激素非依赖的LNCaP-AI细胞过程中,差异表达的microRNA主要调控表皮生长因子受体(EGFR)、基质金属蛋白酶9(MMP-9)、Bcl-2及雄激素相关代谢酶。结论前列腺癌激素非依赖转化过程中存在microRNA的差异表达,可能涉及雄激素受体(AR)旁路信号通路、金属酶、抗凋亡基因及雄激素相关代谢酶基因等。
Objective To compare the expression of microRNA in androgen-independent prostate cancer (LNCaP) cells and androgen-independent prostate cancer cells (LNCaP-AI) and to explore the post-transcriptional regulation of hormone-dependent to non-dependent prostate cancer. Methods The microRNA expression in LNCaP and LNCaP-AI cells was detected by Agilent microarray. Six microRNAs were verified by RT-PCR, and the bioinformatics analysis of the target genes regulated by differentially expressed microRNAs was performed. Results Gene microarray detection showed that compared with LNCaP, LNCaP-AI cells reduced expression of 27 microRNAs and increased expression of 11 microRNAs. Six microRNAs were verified by RT-PCR and the results were consistent with those of the gene chip. Conversion of LNCaP cells into hormone-independent LNCaP-AI cells was found by searching http://www.mir-base.org/ for target genes regulated by microRNAs and by reference to the function of known androgen-independent genes The differentially expressed microRNAs mainly regulate epidermal growth factor receptor (EGFR), matrix metalloproteinase 9 (MMP-9), Bcl-2 and androgen-related metabolic enzymes. Conclusion Differential expression of microRNA in hormone-independent transformation of prostate cancer may involve the androgen receptor (AR) signal pathway, metalloenzyme, anti-apoptotic gene and androgen-related metabolic enzyme genes.