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目的检测类风湿关节炎(RA)患者外周血CCR6+CD4+T细胞芳香烃受体(AhR)水平,探讨AhR在RA发病机制中的意义。方法采用流式细胞术测定33例RA患者及14例健康对照者外周血CCR6+CD4+T细胞中AhR阳性细胞比例,分析其与RA患者临床指标的关系。结果 1)RA患者组外周血单个核细胞(PBMCs)中CCR6+CD4+T细胞占(10.75±2.92)%,高于健康对照组(4.96±2.28)%,差异有统计学意义(t=6.581,P<0.01)。2)RA患者组内外周血CCR6+CD4+T细胞中AhR阳性细胞所占比例(46.02±14.68)%,高于CCR6-CD4+T细胞(21.62±6.94)%和PBMCs(34.22±10.33)%中AhR阳性细胞占比,差异均有统计学意义(z=-6.432,P<0.01;t=-3.776,P<0.01)。3)RA患者组外周血CCR6+CD4+T细胞中AhR阳性细胞所占比例(46.02±14.68)%,高于健康对照组(23.18±5.62)%,差异有统计学意义(z=-4.909,P<0.01)。4)相关性分析:RA患者组外周血CCR6+CD4+T细胞中AhR阳性细胞所占比例与血沉(ESR)、C反应蛋白(CRP)、抗环瓜氨酸肽(抗-CCP)抗体、DAS28评分、疾病病程均无相关性(P均>0.05)。结论 AhR阳性细胞在RA患者外周血CCR6+CD4+T细胞中比例高,可能参与了RA发病,但其水平高低不能反映RA疾病活动程度。
Objective To detect the level of aromatic receptor (AhR) in peripheral blood of patients with rheumatoid arthritis (RA) and to explore the role of AhR in the pathogenesis of RA. Methods Flow cytometry was used to determine the percentage of AhR positive cells in peripheral blood of 33 patients with RA and 14 healthy controls. The correlation between AhR positive cells and clinical features of RA patients was analyzed. Results 1) The percentage of CCR6 + CD4 + T cells in peripheral blood mononuclear cells (PBMCs) in RA patients was (10.75 ± 2.92)%, which was significantly higher than that in healthy controls (4.96 ± 2.28)%, the difference was statistically significant (t = 6.581 , P <0.01). 2) The percentage of AhR positive cells in CCR6 + CD4 + T cells in peripheral blood of RA patients was 46.02 ± 14.68%, higher than that of CCR6-CD4 + T cells (21.62 ± 6.94)% and PBMCs (34.22 ± 10.33)% In the proportion of AhR positive cells, the differences were statistically significant (z = -6.432, P <0.01; t = -3.776, P <0.01). 3) The percentage of AhR positive cells in CCR6 + CD4 + T cells in peripheral blood of RA patients was 46.02 ± 14.68%, which was significantly higher than that of healthy controls (23.18 ± 5.62)%, the difference was statistically significant (z = -4.909, P <0.01). 4) Correlation analysis: The proportion of AhR positive cells in peripheral blood of RA patients was positively correlated with ESR, C-reactive protein (CRP), anti-cyclic citrullinated peptide (anti-CCP) DAS28 score, disease duration had no correlation (P> 0.05). Conclusion The positive rate of AhR positive cells in RA patients with CCR6 + CD4 + T cells is high, which may be involved in the pathogenesis of RA. However, the level of AhR positive cells does not reflect the degree of RA activity.