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用聚合酶链反应（ＰＣＲ）法将Ｃ肽为６个氨基酸残基的胰岛素原类似物基因删除突变成Ｃ肽仅为２个精氨酸残基的胰岛素原类似物（ＢＡｒｇ－Ａｒｇ－Ａ）基因，即把ｐＵＣ１８ＢＣ＇Ａ改建为ｐＵＣ１８ＢＲ＿２Ａ。再将ｐＵＣ１８ＢＲ＿２Ａ与ｐＪＧ１０５重组为表达质粒ｐＪＧ１０７，使Ｂ－Ａｒｇ－Ａｒｇ－Ａ与ｐＪＧ１０５编码的一种多肽构成融合蛋白在大肠杆菌体系中表达。融合蛋白占细菌蛋白总量的５８％。表达产物具有人胰岛素放射免疫活性。
Polymerase chain reaction (PCR) method was used to delete the proinsulin analog of C-peptide of 6 amino acid residues by mutagenesis to proinsulin analogue of only 2 arginine residues of C-peptide (BArg-Arg-A ) Gene, that is, pUC18BC’A was transformed into pUC18BR_2A. The pUC18BR_2A and pJG105 were recombined into the expression plasmid pJG107 to express a polypeptide encoded by B-Arg-Arg-A and pJG105 in Escherichia coli. The fusion protein accounted for 58% of the total bacterial protein. The expression product has human insulin radioimmunoactivity.