论文部分内容阅读
目的观察拟人参皂苷F11(PF11)对甲基苯丙胺(MA)依赖大鼠脑组织中氧化物质的干预,探讨PF11对MA依赖大鼠脑损伤的保护作用。方法采用剂量递增法建立MA依赖大鼠模型,分别ig大鼠3、6 mg·kg-1PF11,连续12 d,第13天断头取血、脑,观察各组大鼠血清及脑组织中谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)、丙二醛(MDA)含量的变化。结果 MA依赖大鼠血清及脑组织海马区MDA的含量显著升高,SOD、GSH-Px的活力下降,与对照组有显著差异;与模型组比较,PF11 6 mg·kg-1组的脑组织海马区MDA的含量显著降低,SOD、GSH-Px的活力显著升高。结论 PF11能降低MA染毒大鼠脑组织海马区MDA的含量,升高SOD、GSH-Px的活力,通过抗氧化作用对脑组织损伤有较好的干预与保护作用。
Objective To observe the effect of PF11 on methamphetamine (MA) -induced oxidative stress in rat brain tissue and to explore the protective effect of PF11 on brain damage in MA-dependent rats. Methods MA-dependent rat model was established by dose escalation. Rats were sacrificed at 3, 6 mg · kg-1 PF11 for 12 days, respectively. On the 13th day, the animals were decapitated to take blood and brain. The changes of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and malondialdehyde (MDA) Results The content of MDA in hippocampus of MA-dependent rats increased significantly, the activity of SOD and GSH-Px decreased significantly compared with the control group. Compared with the model group, the brain tissue of PF11 6 mg · kg-1 group The content of MDA in hippocampus decreased significantly and the activities of SOD and GSH-Px increased significantly. Conclusions PF11 can reduce the content of MDA in the hippocampus and the activity of SOD and GSH-Px in the brain tissue of MA-treated rats. It has better intervention and protection effect on the brain tissue injury through anti-oxidation.