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以醋栗番茄LA722和普通番茄9706为材料,利用发芽期大规模低温胁迫筛选方法和AB-QTL方法对野生醋栗番茄发芽期和幼苗期的耐冷性QTL进行了定位研究,构建了包括273个BC3S1株系的高代回交作图群体,以种子相对发芽指数和苗期耐冷指数作为耐冷指标,检测到5个与发芽期耐冷性相关的QTLs,分别位于第1、2、5、11染色体上,2个与幼苗期耐冷性相关的QTLs,分别位于第2和第8染色体上。其中,控制发芽期耐冷性的qRGI-2-1和qRGI-5-1贡献率分别为12.8%和32.9%。有12个单株的自交后代(BC3S2)在发芽期和幼苗期的耐冷性都达到或优于LA722的水平,且果实质量等农艺性状均接近或优于普通番茄9706,可作为番茄耐冷育种材料。
QTLs for cold tolerance in wild gooseberry tomato at germination and seedling stages were studied using screening methods of low-temperature stress in jujube and AB-QTL, using LA722 and ordinary tomato 9706 as the materials. A total of 273 QTLs Five high-generation QTLs related to cold tolerance in germination were detected by relative germination index and seedling cold tolerance index of BC3S1 lines, which were located on chromosomes 1, 2, 5 and 11, respectively , Two QTLs related to cold tolerance in seedling were located on chromosomes 2 and 8, respectively. Among them, the contribution rates of qRGI-2-1 and qRGI-5-1 controlling the cold tolerance at germination stage were 12.8% and 32.9%, respectively. The cold tolerance of BC3S2 had reached or exceeded the level of LA722 at the germination and seedling stages, and the agronomic traits such as fruit quality were all close to or better than that of common tomato 9706, material.