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目的研究某药物对铅染毒小鼠抗氧化作用的影响。方法用醋酸铅水溶液为小鼠腹腔注射染毒。然后将染毒小鼠分成治疗1组、治疗2组和模型组,另设正常组。治疗1组、治疗2组用不同剂量的某药物灌服治疗。实验结束,从眼球取血,分离血清。处死动物后,取大脑制成10%脑匀浆。测定各组血、脑超氧化物歧化酶(SOD)、脑谷胱甘肽(GSH)、乙酰胆碱酯酶(TCHE)等生化指标。结果血清总SOD活力模型组明显低于其他各组,差异有统计学意义(P<0.01);脑SOD活力正常组和治疗1组明显高于其他两组,差异有统计学意义(P<0.05,P<0.01);脑GSH含量模型组明显高于其他组,差异有统计学意义(P<0.01);脑TCHE活力治疗1组明显低于正常组和治疗2组,差异有统计学意义(P<0.01);脑铅含量模型组明显高于其他组,差异有统计学意义(P<0.01,P<0.05);血清丙氨酸氨基转移酶(ALT)模型组明显高于其他组,差异有统计学意义(P<0.01);血清尿素各组间比较,差异无统计学意义。结论治疗1组和治疗2组的血清SOD、ALT、脑SOD,脑铅和GSH均接近正常组,与不治疗的模型组比较,差异有统计学意义,治疗后小鼠抗氧化能力和肝功能均得到保护。
Objective To study the effect of a drug on antioxidation in lead-exposed mice. Methods The mice were injected intraperitoneally with lead acetate solution. The mice were then divided into treatment group 1, treatment group 2 and model group, another set of normal group. Treatment 1 group, treatment 2 groups with different doses of a drug infusion treatment. The experiment is over, blood is taken from the eye and the serum is separated. After the animals were sacrificed, the brain was made into 10% brain homogenate. The biochemical indexes such as blood, brain SOD, GSH and TCHE in each group were determined. Results The serum total SOD activity in the model group was significantly lower than the other groups (P <0.01). The activity of SOD in the normal group and the treatment group 1 were significantly higher than those in the other two groups (P <0.05 , P <0.01). The content of GSH in brain tissue in model group was significantly higher than that in other groups (P <0.01). The activity of brain TCHE in group 1 was significantly lower than that in normal group and treatment group 2 (P <0.01) P <0.01). The levels of lead in model group were significantly higher than those in other groups (P <0.01, P <0.05), and the levels of serum alanine aminotransferase (ALT) in model group were significantly higher than those in other groups There was statistical significance (P <0.01); There was no significant difference between the groups of serum urea. Conclusion The serum levels of SOD, ALT, brain SOD, brain lead and GSH in treatment group 1 and treatment group 2 are close to those in normal group. There is significant difference between the untreated group and untreated group. After treatment, the antioxidant capacity and liver function Are protected.